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HiMedia Laboratories macconkey agar medium
Macconkey Agar Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macconkey+agar+medium/macconkey+agar/pm40367070-78-17-20
Average 90 stars, based on 1 article reviews
macconkey agar medium - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: Epidemiology and molecular characterisation of multidrug-resistant Escherichia coli isolated from chicken meat.
Article Snippet: .. Isolation and presumptive detection of E. coli To isolate E. coli, the enriched culture was streaked onto MacConkey agar medium (HIMEDIA, pH: 7.1 ± 0.2, Mumbai, India) and incubated at 37°C for 24 hours. ..

Article Title: Molecular Detection of Tetracycline-Resistant Genes in Multi-Drug-Resistant Escherichia coli Isolated from Broiler Meat in Bangladesh.
Article Snippet: .. Isolation and Identification of E. coli The enriched culture was streaked on a MacConkey agar medium (HIMEDIA) and incubated at 37 °C for 24 h. The colonies demonstrating appropriate E. coli morphology Figure 1. ..

Article Title: Molecular Detection of Tetracycline-Resistant Genes in Multi-Drug-Resistant Escherichia coli Isolated from Broiler Meat in Bangladesh.
Article Snippet: The samples were diced and transferred to separate sterile test tubes containing buffered peptone water (BPW; HIMEDIA, Mumbai, India) and incubated at 37 ◦C overnight for primary enrichment. .. Isolation and Identification of E. coli The enriched culture was streaked on a MacConkey agar medium (HIMEDIA) and incubated at 37 ◦C for 24 h. The colonies demonstrating appropriate E. coli morphology were streaked on eosin methylene blue (EMB) agar plates (Merck, Rahway, NJ, USA) and incubated at 37 ◦C for 24 h for the confirmation of colony morphology. ..

Incubation:

Article Title: Epidemiology and molecular characterisation of multidrug-resistant Escherichia coli isolated from chicken meat.
Article Snippet: .. Isolation and presumptive detection of E. coli To isolate E. coli, the enriched culture was streaked onto MacConkey agar medium (HIMEDIA, pH: 7.1 ± 0.2, Mumbai, India) and incubated at 37°C for 24 hours. ..

Article Title: Molecular Detection of Tetracycline-Resistant Genes in Multi-Drug-Resistant Escherichia coli Isolated from Broiler Meat in Bangladesh.
Article Snippet: .. Isolation and Identification of E. coli The enriched culture was streaked on a MacConkey agar medium (HIMEDIA) and incubated at 37 °C for 24 h. The colonies demonstrating appropriate E. coli morphology Figure 1. ..

Article Title: Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh
Article Snippet: .. The sample was filtered through a 0.45-μm membrane filter, and subsequently, the filter was transferred into a buffer peptone water medium (Himedia, India) for enrichment at 37°C for 24 h. The enriched culture was then subcultured onto MacConkey agar medium (Himedia, India) and incubated at 37°C for 24 h. The large, mucoid, and pink colonies were identified as Klebsiella quasipneumoniae after extraction of DNA ( ) followed by the amplification in a multiplex PCR scheme using the primers OKP-F (5′-GGCCGGYGAGCGGGGCTCA-3′) and DeoR-R (5′- AGAAGCATCCTGCTGTGCG -3′) ( ). .. Following Clinical and Laboratory Standards Institute (CLSI) guidelines , an antibiotic susceptibility test was carried out by disc diffusion method employing available antibiotics (Oxoid, United Kingdom).

Article Title: Molecular Detection of Tetracycline-Resistant Genes in Multi-Drug-Resistant Escherichia coli Isolated from Broiler Meat in Bangladesh.
Article Snippet: The samples were diced and transferred to separate sterile test tubes containing buffered peptone water (BPW; HIMEDIA, Mumbai, India) and incubated at 37 ◦C overnight for primary enrichment. .. Isolation and Identification of E. coli The enriched culture was streaked on a MacConkey agar medium (HIMEDIA) and incubated at 37 ◦C for 24 h. The colonies demonstrating appropriate E. coli morphology were streaked on eosin methylene blue (EMB) agar plates (Merck, Rahway, NJ, USA) and incubated at 37 ◦C for 24 h for the confirmation of colony morphology. ..

Article Title: Molecular Detection of Tetracycline-Resistant Genes in Multi-Drug-Resistant Escherichia coli Isolated from Broiler Meat in Bangladesh
Article Snippet: The samples were diced and transferred to separate sterile test tubes containing buffered peptone water (BPW; HIMEDIA, Mumbai, India) and incubated at 37 °C overnight for primary enrichment. .. The enriched culture was streaked on a MacConkey agar medium (HIMEDIA) and incubated at 37 °C for 24 h. The colonies demonstrating appropriate E. coli morphology were streaked on eosin methylene blue (EMB) agar plates (Merck, Rahway, NJ, USA) and incubated at 37 °C for 24 h for the confirmation of colony morphology. ..

Article Title: Design and Fabrication of Broad-Spectrum Antimicrobial Porous Metallo-Polymeric Microsphere for Water Disinfection.
Article Snippet: An expedient and efficient approach is used to synthesize a new class of metallo-polymeric microspheres (MPMs) as antimicrobials to succumb the wide range of bacteria from water.. Three types of MPMs, that is, poly[Silver (I)-methacrylate-co-methylmethacrylate] (pAgMA), poly[Copper (II)-methacrylate-co-methyl methacrylate] (pCuMA), and poly[Nickel (II)-methacrylate-co-methylmethacrylate] (pNiMA), are prepared via radical suspension polymerization technique in 3D shape with porous texture.. The structural and morphological characterization of the prepared microspheres are examined by analytical techniques.

Article Title: Epidemiology and molecular characterisation of multidrug-resistant Escherichia coli isolated from chicken meat
Article Snippet: .. To isolate E. coli , the enriched culture was streaked onto MacConkey agar medium (HIMEDIA, pH: 7.1 ± 0.2, Mumbai, India) and incubated at 37°C for 24 hours. ..

Membrane:

Article Title: Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh
Article Snippet: .. The sample was filtered through a 0.45-μm membrane filter, and subsequently, the filter was transferred into a buffer peptone water medium (Himedia, India) for enrichment at 37°C for 24 h. The enriched culture was then subcultured onto MacConkey agar medium (Himedia, India) and incubated at 37°C for 24 h. The large, mucoid, and pink colonies were identified as Klebsiella quasipneumoniae after extraction of DNA ( ) followed by the amplification in a multiplex PCR scheme using the primers OKP-F (5′-GGCCGGYGAGCGGGGCTCA-3′) and DeoR-R (5′- AGAAGCATCCTGCTGTGCG -3′) ( ). .. Following Clinical and Laboratory Standards Institute (CLSI) guidelines , an antibiotic susceptibility test was carried out by disc diffusion method employing available antibiotics (Oxoid, United Kingdom).

Extraction:

Article Title: Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh
Article Snippet: .. The sample was filtered through a 0.45-μm membrane filter, and subsequently, the filter was transferred into a buffer peptone water medium (Himedia, India) for enrichment at 37°C for 24 h. The enriched culture was then subcultured onto MacConkey agar medium (Himedia, India) and incubated at 37°C for 24 h. The large, mucoid, and pink colonies were identified as Klebsiella quasipneumoniae after extraction of DNA ( ) followed by the amplification in a multiplex PCR scheme using the primers OKP-F (5′-GGCCGGYGAGCGGGGCTCA-3′) and DeoR-R (5′- AGAAGCATCCTGCTGTGCG -3′) ( ). .. Following Clinical and Laboratory Standards Institute (CLSI) guidelines , an antibiotic susceptibility test was carried out by disc diffusion method employing available antibiotics (Oxoid, United Kingdom).

Amplification:

Article Title: Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh
Article Snippet: .. The sample was filtered through a 0.45-μm membrane filter, and subsequently, the filter was transferred into a buffer peptone water medium (Himedia, India) for enrichment at 37°C for 24 h. The enriched culture was then subcultured onto MacConkey agar medium (Himedia, India) and incubated at 37°C for 24 h. The large, mucoid, and pink colonies were identified as Klebsiella quasipneumoniae after extraction of DNA ( ) followed by the amplification in a multiplex PCR scheme using the primers OKP-F (5′-GGCCGGYGAGCGGGGCTCA-3′) and DeoR-R (5′- AGAAGCATCCTGCTGTGCG -3′) ( ). .. Following Clinical and Laboratory Standards Institute (CLSI) guidelines , an antibiotic susceptibility test was carried out by disc diffusion method employing available antibiotics (Oxoid, United Kingdom).

Multiplex Assay:

Article Title: Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh
Article Snippet: .. The sample was filtered through a 0.45-μm membrane filter, and subsequently, the filter was transferred into a buffer peptone water medium (Himedia, India) for enrichment at 37°C for 24 h. The enriched culture was then subcultured onto MacConkey agar medium (Himedia, India) and incubated at 37°C for 24 h. The large, mucoid, and pink colonies were identified as Klebsiella quasipneumoniae after extraction of DNA ( ) followed by the amplification in a multiplex PCR scheme using the primers OKP-F (5′-GGCCGGYGAGCGGGGCTCA-3′) and DeoR-R (5′- AGAAGCATCCTGCTGTGCG -3′) ( ). .. Following Clinical and Laboratory Standards Institute (CLSI) guidelines , an antibiotic susceptibility test was carried out by disc diffusion method employing available antibiotics (Oxoid, United Kingdom).

Polymerase Chain Reaction:

Article Title: Draft Genome Sequence of a Multidrug-Resistant and Novel Sequence Type 6130 Klebsiella quasipneumoniae Strain C11S11_BCSIR Isolated from Wastewater of a Tertiary Care Hospital in Chattogram, Bangladesh
Article Snippet: .. The sample was filtered through a 0.45-μm membrane filter, and subsequently, the filter was transferred into a buffer peptone water medium (Himedia, India) for enrichment at 37°C for 24 h. The enriched culture was then subcultured onto MacConkey agar medium (Himedia, India) and incubated at 37°C for 24 h. The large, mucoid, and pink colonies were identified as Klebsiella quasipneumoniae after extraction of DNA ( ) followed by the amplification in a multiplex PCR scheme using the primers OKP-F (5′-GGCCGGYGAGCGGGGCTCA-3′) and DeoR-R (5′- AGAAGCATCCTGCTGTGCG -3′) ( ). .. Following Clinical and Laboratory Standards Institute (CLSI) guidelines , an antibiotic susceptibility test was carried out by disc diffusion method employing available antibiotics (Oxoid, United Kingdom).

Bacteria:

Article Title: Design and Fabrication of Broad-Spectrum Antimicrobial Porous Metallo-Polymeric Microsphere for Water Disinfection.
Article Snippet: An expedient and efficient approach is used to synthesize a new class of metallo-polymeric microspheres (MPMs) as antimicrobials to succumb the wide range of bacteria from water.. Three types of MPMs, that is, poly[Silver (I)-methacrylate-co-methylmethacrylate] (pAgMA), poly[Copper (II)-methacrylate-co-methyl methacrylate] (pCuMA), and poly[Nickel (II)-methacrylate-co-methylmethacrylate] (pNiMA), are prepared via radical suspension polymerization technique in 3D shape with porous texture.. The structural and morphological characterization of the prepared microspheres are examined by analytical techniques.

Cell Culture:

Article Title: Design and Fabrication of Broad-Spectrum Antimicrobial Porous Metallo-Polymeric Microsphere for Water Disinfection.
Article Snippet: An expedient and efficient approach is used to synthesize a new class of metallo-polymeric microspheres (MPMs) as antimicrobials to succumb the wide range of bacteria from water.. Three types of MPMs, that is, poly[Silver (I)-methacrylate-co-methylmethacrylate] (pAgMA), poly[Copper (II)-methacrylate-co-methyl methacrylate] (pCuMA), and poly[Nickel (II)-methacrylate-co-methylmethacrylate] (pNiMA), are prepared via radical suspension polymerization technique in 3D shape with porous texture.. The structural and morphological characterization of the prepared microspheres are examined by analytical techniques.



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Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in <t>MacConkey</t> media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).
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Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in MacConkey media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).

Journal: Gut Microbes Reports

Article Title: The living medicine inside us: in vitro therapeutic prospects of human gut bacteria

doi: 10.1080/29933935.2025.2480093

Figure Lengend Snippet: Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in MacConkey media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).

Article Snippet: These are Bacterial Growth Medium (BGM), Brain Heart Infusion (BHI) Medium (Condalab, Madrid, Spain) and MacConkey Agar Medium (Condalab, Madrid, Spain).

Techniques: Isolation, Bacteria, Amplification, Positive Control, Negative Control